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COBEM 2023

27th International Congress of Mechanical Engineering

MICROALGAL BIOMASS PRODUCTION OF “SCENEDESMUS MIX” THROUGH HETEROTROPHIC CULTIVATION

Submission Author: Anne Defranceschi Oliveira , PR , Brazil
Co-Authors: Anne Defranceschi Oliveira, Eduardo Sassi carocci, Flávio Júnior Santiago Silva, Iago Gomes Costa, Murilo Gasparin Rampi, Diego de Lima Sousa, JOSÉ VIRIATO COELHO VARGAS, Vanessa Kava, André Bellin Mariano
Presenter: Anne Defranceschi Oliveira

doi://10.26678/ABCM.COBEM2023.COB2023-1528

 

Abstract

Microalgae are microscopic photosynthetic organisms with generally simple nutritional needs. In recent times, the production of microalgae has gained a new focus, production aimed at energy development due to some characteristics present in its biomass. The microalgal biomass can contain from 1 to 70% of lipids, which aroused the interest of research focused on the production of biodiesel. Microalgae are micro-organisms capable of presenting three distinct metabolisms against the source of carbon available for growth: the autotrophic metabolism, where in the presence of light and certain mineral salts the microalgae is capable of fixing carbon dioxide; heterotrophic metabolism, where the microalgae need an organic carbon source present in the culture medium in the absence of light, and mixotrophic metabolism, where the two previous metabolic forms are developed together. This study aimed to optimize biomass production through heterotrophic cultivation of a mix of microalgae from the genus Scenedesmus, using an experimental design technique. Variables such as the composition of the culture medium (glucose, sodium nitrate, and sodium phosphate dibasic) were chosen and evaluated in three levels to observe their effect on biomass production. Results showed that glucose had the largest effect on biomass production, resulting in an increase of 11.215 gL-1 of biomass when the level proposed in the design was used. The variable sodium phosphate had an effect of 1.215 gL-1, but it was not statistically significant. The variable sodium nitrate had a negative effect when set at the high level, resulting in a decrease of -1.765 gL-1. However, determining the optimal value of this variable could lead to better results. In conclusion, the use of heterotrophic cultivation for the production of biomass from the Scenedesmus mix may be an appropriate practice for producing microalgal biomass, resulting in yields greater than those obtained through optimal cultivation of other parameters not determined in this experiment.

Keywords

HETEROTROPHIC, Biomass, microalgae

 

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