Eventos Anais de eventos
COBEM 2023
27th International Congress of Mechanical Engineering
Sensitivity of Donnan’s model parameters to the modeling of osmotic pressure in tendons
Submission Author:
Thiago André Carniel , SC
Co-Authors:
Eduarda Bordignon Atuatti, João Paulo Eckert, Thiago André Carniel, Liz Girardi Müller, Samara Cristina Mazon, Micheli Zanetti, Éverton Breitenbach, Márcio Antônio Fiori, Rafael Geronimo, Bruno Klahr, José Luís Medeiros Thiesen, Otávio Teixeira Pinto, Eduardo Fancello
Presenter: Eduarda Bordignon Atuatti
doi://10.26678/ABCM.COBEM2023.COB2023-0788
Abstract
Tendons are fibrous connective tissues that contain a significant amount of water in their composition. However, the specific role of water in mechanical, chemical and biological functions has not been clearly stated in the literature, requiring further research. In this context, the aim of this study is to investigate the extent to which the parameters of the well-known Donnan’s model affect the overall osmotic pressure within tendon tissues. These parameters include temperature, fluid volume fraction, external chemical concentration and fixed charge density (FCD). In order to establish reference values for each of these parameters, a literature survey and a series of in vitro experiments were performed. Firstly, a range of temperatures that tendons can experience were obtained from previously published in vivo experiments. Secondly, the fluid volume fraction was characterized using a water content protocol combined with a digital image segmentation approach. Thirdly, the external chemical concentration was measured from a specific polyethylene glycol buffer solution, designed to mimic the in vivo chemical environment of tendons. Lastly, the fixed charge density was estimated based on the total sulfated glycosaminoglycans (GAG) content, which was quantified through a biochemical assay (Dimethylmethylene Blue Assay – DMMB Assay). All in vitro experiments were performed on bovine tendons. The achieved results are preliminary in nature and are intended to contribute to future research in this field using numerical simulations based on biphasic-swelling models.
Keywords
Tendon, Osmosis, Interstitial fluid, Poroelastic, Donnan’s model
