Eventos Anais de eventos
COBEM 2023
27th International Congress of Mechanical Engineering
EXPERIMENTAL STUDY ON VITRIFICATION OF CRYOPROTECTANTS USING THIN-FILM EVAPORATION OF NITROGEN
Submission Author:
Henrique Vidaletti , RS
Co-Authors:
Henrique Vidaletti, Álisson Renan Stochero da Silva, Jacqueline Copetti, Mario Henrique Macagnan, Jeferson Diehl de Oliveira, Elaine Maria Cardoso, Leonardo Manetti
Presenter: Álisson Renan Stochero da Silva
doi://10.26678/ABCM.COBEM2023.COB2023-0720
Abstract
Nitrogen thin film evaporation is a novel technique applied to cryopreservation, which aims to improve the preservation of biological samples at ultra-low temperatures. Cryopreservation is a critical process used in various fields, including biomedical research, regenerative medicine, and the storage of genetic materials. Traditional cryopreservation methods often suffer from sample damage caused by ice crystal formation and chemical toxicity associated with conventional cryoprotectants. This study developed an experimental apparatus comprising a vacuum chamber and two cryocauteries. The sample is stored in a PDMS reservoir between two copper plates with a nickel microporous outer surface. This system is suspended inside the chamber, and nitrogen jets promote evaporation. Chamber pressure and sample temperatures are recorded during the experiments. Tests were performed using DMSO, glycerol and sucrose solutions in PBS at different concentrations and volumes to evaluate vitrification and cooling rates. Effects of volume and concentration were evaluated, and it was found that vitrification success is directly related to the CPA concentration used. In addition, it was not observed any significant impact on the visual appearance of the samples related to vitrification regarding the sample geometry. The copper foam porous surface resulted in the highest cooling rate, which is associated with the properties of the foam, such as its high thermal conductivity.
Keywords
evaporation heat transfer, cell vitrification, liquid nitrogen, Cryopreservation
