Eventos Anais de eventos
COBEM 2021
26th International Congress of Mechanical Engineering
COMPARATIVE STUDY OF BIOGENIC AND CHEMICAL SYNTHESIZED HYDROXYAPATITE FOR BIOMEDICAL APPLICATIONS
Submission Author:
Marla Horta , RJ
Co-Authors:
Marla Horta, Francisco José Moura, MARILZA AGUILAR, Jose Brant de Campos, Daniel Navarro da Rocha, Cecília Westin
Presenter: Marla Horta
doi://10.26678/ABCM.COBEM2021.COB2021-2154
Abstract
The use of biogenic sources to obtain calcium phosphate compounds, mainly hydroxyapatite (HAp), is an excellent alternative to try to reduce costs in obtaining biomaterials. The purpose of the present study was to compare hydroxyapatite obtained from commercial calcium source and from Colossoma macropomum fish bone through the physical-chemical characteristics and in vitro tests. The materials were characterized by X ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR). The sample were treated at 800°C and the XRD results indicated the presence of only hydroxyapatite for the samples obtained from fish bones indicating the purity and stability of the material. The FTIR analysis showed the presence of various functional groups such as PO43-, CO32- and OH- corresponding to HAp. SEM observation confirmed the morphology of the samples to be rod-like and spherical-like. The cell viability and bioactivity of the materials were evaluated by testing the reduction of rezasurin and McCoy medium assay, respectively. The materials did not have a toxic effect and the sample obtained from the fish bone was more bioactive compared to hydroxyapatite from commercial calcium source. It was found that natural HAp exhibited better structural properties compared with synthetic HAp. Therefore, the bio-wastes from fish bone can be utilized in the obtainment of hydroxyapatite which can serve as an alternative for the conventional chemical method getting HAp with good properties and promising application in the biomedical area .
Keywords
Hydroxyapatite, fish bone, biomaterial, in vitro assay, biowaste
