Eventos Anais de eventos
COBEM 2021
26th International Congress of Mechanical Engineering
NUMERICAL-EXPERIMENTAL INVESTIGATION OF CRYOPRESERVATION PROCESS BY DROPLET VITRIFICATION
Submission Author:
Álisson Renan Stochero da Silva , RS
Co-Authors:
Álisson Renan Stochero da Silva, Jacqueline Copetti, Mario Henrique Macagnan, Guilherme Steffenon, Massoxi Cuiêca
Presenter: Álisson Renan Stochero da Silva
doi://10.26678/ABCM.COBEM2021.COB2021-1255
Abstract
The vitrification of biological materials is a segment of cryopreservation that aims the ultrafast freezing of a cell sample, through the direct transition from a liquid state to a vitrified and amorphous state, without the occurrence of crystallization, reaching cooling rates in the order of 37.000 °C/s, depending on the type of protocol to be used. Several studies have emerged in order to develop and improve ultrafast freezing protocols by vitrification, as well as to investigate the governing phenomena of these processes, both in the field of assisted reproduction and in the study of stem cells for the treatment of diseases. In this context, this work aims to investigate numerically and experimentally the phenomena involved during the vitrification process, such as cooling rate, solidification rate and temperature behavior. There were performed 6 experiments obtaining the curves of temperature and values of cooling rates. A good agreement between the numerical result and the experimental results can be seen, with a relative difference of 0.33% for the cooling rate, being 589.92 °C/min and 591.8 °C/min, respectively. The total solidification of the droplet occurred in 9.965 seconds. The maximum difference between the average of the experimental and numerical cooling rate was 30.54%, due to the temperature measurement position and the discrepancy between the experimental and simulated contact angles.
Keywords
Cryopreservation, Droplet Vitrification, Superflash Freezing, Computational Fluid Dynamics
